Plasmid

Part:BBa_K4202018:Design

Designed by: ZIHANG YE   Group: iGEM22_ZJU-China   (2022-10-01)


This is a expression vector for the protein expression in Bacillus subtilis.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 530
    Illegal XbaI site found at 6
    Illegal PstI site found at 15
    Illegal PstI site found at 4323
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 530
    Illegal PstI site found at 15
    Illegal PstI site found at 4323
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 530
    Illegal BglII site found at 11
    Illegal BamHI site found at 339
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 530
    Illegal XbaI site found at 6
    Illegal PstI site found at 15
    Illegal PstI site found at 4323
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 530
    Illegal XbaI site found at 6
    Illegal PstI site found at 15
    Illegal PstI site found at 4323
    Illegal AgeI site found at 5202
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3080
    Illegal BsaI site found at 4497


Design Notes

We recommend using the infusion cloning strategy for joining CDS


Source

We obtain the PHY300PLK(BBa_K4202016) form FENGHIUSHNENGWU company and the sequence of P3 promotor and double terminator are obtained from the distribution part from iGEM by PCR.

References